Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Opt Lett. 2009 Mar 1;34(5):653-5. doi: 10.1364/ol.34.000653.


We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 microm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.

MeSH terms

  • Cells / ultrastructure*
  • Equipment Design
  • Erythrocyte Membrane / ultrastructure
  • Holography / instrumentation
  • Holography / methods
  • Humans
  • Microscopy / instrumentation
  • Microscopy / methods*
  • Tomography, Optical / instrumentation
  • Tomography, Optical / methods*