Two-dimensional proteome reference maps for the human pathogenic filamentous fungus Aspergillus fumigatus

Proteomics. 2009 Mar;9(5):1407-15. doi: 10.1002/pmic.200800394.


The filamentous fungus Aspergillus fumigatus has become the most important airborne fungal pathogen causing life-threatening infections in immunosuppressed patients. We established a 2-D reference map for A. fumigatus. Using MALDI-TOF-MS/MS, we identified 381 spots representing 334 proteins. Proteins involved in cellular metabolism, protein synthesis, transport processes and cell cycle were most abundant. Furthermore, we established a protocol for the isolation of mitochondria of A. fumigatus and developed a mitochondrial proteome reference map. 147 proteins represented by 234 spots were identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus fumigatus / cytology
  • Aspergillus fumigatus / metabolism*
  • Cell Cycle
  • Fungal Proteins / analysis*
  • Fungal Proteins / metabolism
  • Humans
  • Mitochondria / metabolism
  • Protein Biosynthesis
  • Proteome / analysis*
  • Proteome / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tandem Mass Spectrometry


  • Fungal Proteins
  • Proteome