Extracellular chitinases of mutant superproducing strain Serratia marcescens M-1

A B Duzhak; Z I Panfilova; T G Duzhak; E A Vasyunina

doi:10.1134/s0006297909020126

Extracellular chitinases of mutant superproducing strain Serratia marcescens M-1

Biochemistry (Mosc). 2009 Feb;74(2):209-14. doi: 10.1134/s0006297909020126.

Authors

A B Duzhak¹, Z I Panfilova, T G Duzhak, E A Vasyunina

Affiliation

¹ Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. abduzhak@mail.ru

PMID: 19267677
DOI: 10.1134/s0006297909020126

Abstract

Four extracellular proteins with chitinase activity capable of binding chitin substrates have been revealed in the culture liquid of chitinase superproducing mutant strain M-1 of Serratia marcescens. Proteins were analyzed by SDS-PAGE and MALDI-TOF mass spectrometry. Based on the data obtained, the proteins were identified as typical chitinases of S. marcescens: ChiA, ChiB, ChiC, and CBP21.

MeSH terms

Bacterial Proteins / biosynthesis
Bacterial Proteins / chemistry*
Bacterial Proteins / isolation & purification
Chitin / metabolism
Chitinases / biosynthesis
Chitinases / chemistry*
Chitinases / isolation & purification
Culture Media
Electrophoresis, Polyacrylamide Gel
Extracellular Space / enzymology
Protein Binding
Serratia marcescens / enzymology*
Serratia marcescens / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Bacterial Proteins
Culture Media
Chitin
Chitinases