Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes

Nancy L Thompson; Xiang Wang; Punya Navaratnarajah

doi:10.1016/j.jsb.2009.02.013

Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes

J Struct Biol. 2009 Oct;168(1):95-106. doi: 10.1016/j.jsb.2009.02.013. Epub 2009 Mar 6.

Authors

Nancy L Thompson¹, Xiang Wang, Punya Navaratnarajah

Affiliation

¹ Department of Chemistry, University of North Carolina at Chapel Hill, 27599-3290, USA. nlt@unc.edu

Abstract

In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cell Membrane / chemistry
Membranes, Artificial*
Models, Theoretical
Spectrometry, Fluorescence / methods*
Surface Plasmon Resonance

Substances

Membranes, Artificial

Abstract

Publication types

MeSH terms

Substances

Grants and funding