Quantitative analysis of volatile selenium metabolites in normal urine by headspace solid phase microextraction gas chromatography-inductively coupled plasma mass spectrometry

Talanta. 2009 May 15;78(3):759-63. doi: 10.1016/j.talanta.2008.12.044. Epub 2008 Dec 30.

Abstract

The combination of headspace-solid phase microextraction (HS-SPME) and gas chromatography-inductively coupled plasma mass spectrometry (GC-ICPMS) was evaluated for the determination of volatile selenium metabolites in normal urine samples, i.e. without selenium supplementation. HS-SPME operating conditions were optimised and a sampling time of 10 min was found to be suitable for simultaneous extraction of dimethylselenide (DMSe) and dimethyldiselenide (DMDSe). The amount of DMSe and DMDSe extracted onto fibre coating was calculated in clean matrix, i.e. Milli-Q water, on the basis of depletion experiments. When applied to normal urine samples, the developed method allowed the detection of four volatile selenium containing species, among which DMSe and DMDSe could be quantified by standard additions.

Publication types

  • Evaluation Study

MeSH terms

  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Organoselenium Compounds / analysis
  • Organoselenium Compounds / urine
  • Selenium / metabolism*
  • Selenium / urine*
  • Solid Phase Microextraction

Substances

  • Organoselenium Compounds
  • dimethyldiselenide
  • Selenium
  • dimethylselenide