Anti-aging effect of rice wine in cultured human fibroblasts and keratinocytes

J Biosci Bioeng. 2009 Mar;107(3):266-71. doi: 10.1016/j.jbiosc.2008.11.016.


The application of rice wine on skin is known to have beneficial effects such as enhancement of the skin barrier function and skin whitening. In this study, we focused on examination of the anti-aging effects of rice wine. The treatment of fibroblasts with rice wine in vitro increased the expression of procollagen and laminin-5, a key basement membrane component in cultured human fibroblasts. Rice wine significantly reduced the expression of UV-induced matrix metalloproteinase-1 (MMP-1) in a dose-dependent manner in both cultured human fibroblasts and keratinocytes. In addition, treatment with rice wine decreased UV-induced tumor necrosis factor-alpha (TNF-alpha) production in human keratinocytes. An in vivo study using hairless mice showed that topical application of rice wine protected mouse skin from photoaging. Thus, we suggest that rice wine may have potential as an effective agent for the prevention and treatment of UV-induced skin aging.

MeSH terms

  • Animals
  • Cells, Cultured
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Fibroblasts / radiation effects
  • Humans
  • Keratinocytes / drug effects*
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects
  • Laminin / metabolism
  • Matrix Metalloproteinase 1 / biosynthesis
  • Mice
  • Mice, Hairless
  • Oryza*
  • Procollagen / metabolism
  • Skin Aging / drug effects*
  • Skin Aging / radiation effects
  • Tumor Necrosis Factor-alpha / metabolism
  • Ultraviolet Rays / adverse effects
  • Wine*


  • Laminin
  • Procollagen
  • Tumor Necrosis Factor-alpha
  • Matrix Metalloproteinase 1