In vivo microCT quantification of lung tumor growth in SPC-raf transgenic mice

Front Biosci (Landmark Ed). 2009 Jan 1;14:1939-44. doi: 10.2741/3353.

Abstract

Lung cancer is the most common cancer worldwide. Early detection might reduce morbidity. In this study we evaluate a microCT imaging algorithm to assess in-vivo tumour load and quantification of tumour growth in a transgenic disease model of lung adenocarcinomas. MicroCT was carried out with n=10 SPC-raf transgenic mice without gating in spontaneously breathing and isoflurane anaesthetised animals. Segmentation of the air-filled spaces was obtained using a region growing algorithm by 3 independent observers. Inter- and intra-observer variability of the algorithm was determined and compared against an alternative region growing algorithm. Due to the multiple very small tumor nodules that occur and the low signal-to-noise ratio direct volumetric measurement of solitary tumor nodules is not feasible. However, tumor growth can be assessed by measuring the decrease in the segmented volume of the aerated lung areas. The presented algorithm can thus be used to evaluate therapeutic efficacies of novel treatment strategies. The imaging algorithm allows in vivo quantification of lung tumor load and tumor growth in transgenic mice with an acceptable intra- and interobserver variability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division
  • Intercellular Signaling Peptides and Proteins
  • Lung Neoplasms / diagnostic imaging
  • Lung Neoplasms / pathology*
  • Mice
  • Mice, Transgenic
  • Peptides / genetics*
  • Tomography, X-Ray Computed / methods*
  • raf Kinases / genetics*

Substances

  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • Sftpc protein, mouse
  • raf Kinases