Resveratrol inhibits the expression of SREBP1 in cell model of steatosis via Sirt1-FOXO1 signaling pathway

Biochem Biophys Res Commun. 2009 Mar 13;380(3):644-9. doi: 10.1016/j.bbrc.2009.01.163. Epub 2009 Jan 31.

Abstract

Recent studies in mice have shown that resveratrol can protect the liver from fat accumulation induced by high fat diet. However, the exact mechanism is largely unknown. To explore the possible mechanism, we investigated the anti-lipogenic effect of resveratrol in vitro model. Oil Red O staining revealed that resveratrol could significantly ameliorate the excessive triglyceride accumulation in HepG2 cells induced by palmitate. The results of RT-PCR and Western blotting showed that resveratrol upregulated the expression of Sirt1 and forkhead box O1 (FOXO1), whereas downregulated the expression of sterol regulatory element binding protein1 (SREBP1). Moreover, resveratrol was shown to inhibit the activity of SREBP1, as evaluated by immunofluorescence assay. Our results suggest that resveratrol may attenuate fat deposition by inhibiting SREBP1 expression via Sirt1-FOXO1 pathway and thus may have application for the treatment of NAFLD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caloric Restriction
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Fatty Liver / metabolism*
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors / metabolism
  • Humans
  • Hypolipidemic Agents / pharmacology*
  • Mice
  • Models, Biological
  • Palmitic Acid / pharmacology
  • Resveratrol
  • Signal Transduction
  • Sirtuin 1
  • Sirtuins / metabolism
  • Sterol Regulatory Element Binding Protein 1 / antagonists & inhibitors*
  • Sterol Regulatory Element Binding Protein 1 / biosynthesis
  • Stilbenes / pharmacology*
  • Triglycerides / metabolism*

Substances

  • FOXO1 protein, human
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors
  • Hypolipidemic Agents
  • SREBF1 protein, human
  • Sterol Regulatory Element Binding Protein 1
  • Stilbenes
  • Triglycerides
  • Palmitic Acid
  • SIRT1 protein, human
  • Sirtuin 1
  • Sirtuins
  • Resveratrol