Plant Rho-type (Rop) GTPase-dependent activation of receptor-like cytoplasmic kinases in vitro

FEBS Lett. 2009 Apr 2;583(7):1175-82. doi: 10.1016/j.febslet.2009.02.047. Epub 2009 Mar 12.


Plants have evolved distinct mechanisms to link Rho-type (Rop) GTPases to downstream signaling pathways as compared to other eukaryotes. Here, experimental data are provided that members of the Medicago, as well as Arabidopsis, receptor-like cytoplasmic kinase family (RLCK Class VI) were strongly and specifically activated by GTP-bound Rop GTPases in vitro. Deletion analysis indicated that the residues implicated in the interaction might be distributed on various parts of the kinases. Using a chimaeric Rop GTPase protein, the importance of the Rho-insert region in kinase activation could also be verified. These data strengthen the possibility that RLCKs may serve as Rop GTPase effectors in planta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Enzyme Activation / physiology
  • Medicago truncatula / enzymology*
  • Medicago truncatula / genetics
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • rho GTP-Binding Proteins / genetics
  • rho GTP-Binding Proteins / metabolism*


  • Arabidopsis Proteins
  • Recombinant Fusion Proteins
  • Protein Kinases
  • rho GTP-Binding Proteins