The human ovarian follicular fluid level of interleukin-8 is associated with follicular size and patient age

Fertil Steril. 2010 Feb;93(2):537-43. doi: 10.1016/j.fertnstert.2008.11.033. Epub 2009 Mar 14.


Objective: To investigate the relationship between interleukin-8 (IL-8) in the human ovarian follicle and follicular size, patient age, and fertility factors in IVF cycles.

Design: Prospective study.

Setting: University hospital research laboratory and infertility clinic.

Patient(s): Women undergoing IVF with oocyte retrieval.

Intervention(s): Follicular fluid (FF) aspiration, oocyte isolation, FF storage, and experimental studies.

Main outcome measure(s): Quantization of IL-8 by ELISAs and protein microarray; high-performance liquid chromatography (HPLC) followed by ELISA and Western blotting to evaluate alpha(2)-macroglobulin (alpha(2)M) bound IL-8; association of IL -8 to follicular size, patient age, and IVF outcomes.

Result(s): Samples of FF from 63 patients contained an average of 629.59 pg/mL of IL-8 with 50%-70% bound to alpha(2)M. Large follicles contained higher levels of IL-8 than small follicles (937.34 vs. 86.97 pg/mL). The IL-8 concentration in the large follicles of women of young age was higher than that of older reproductive age women (1,373.61 vs. 673.29 pg/mL). There were no statistically significant associations found between IL-8 concentration and other IVF cycle factors or pregnancy outcome.

Conclusion(s): Our findings indicate that IL-8 is present in FF, both in its free and alpha(2)M-bound state, and its concentration is correlated with follicular size and patient age.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aging / physiology
  • Body Mass Index
  • Embryo Transfer
  • Enzyme-Linked Immunosorbent Assay
  • Estradiol / blood
  • Female
  • Fertilization / physiology
  • Follicular Fluid / chemistry*
  • Follicular Fluid / physiology
  • Humans
  • Interleukin-8 / analysis*
  • Interleukin-8 / genetics
  • Male
  • Ovarian Follicle / anatomy & histology*
  • Ovarian Follicle / growth & development
  • Ovarian Follicle / physiology
  • Parity
  • Pregnancy
  • Pregnancy Rate
  • Protein Array Analysis
  • Young Adult
  • alpha-Macroglobulins / analysis
  • alpha-Macroglobulins / genetics


  • Interleukin-8
  • alpha-Macroglobulins
  • Estradiol