CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro

Cancer Lett. 2009 Jul 18;280(1):65-71. doi: 10.1016/j.canlet.2009.02.005. Epub 2009 Mar 14.


Stem cells have been found to be involved in breast cancer growth, but the specific contribution of cancer stem cells in tumor biology, including metastasis, is still uncertain. We found that murine breast cancer cell lines 4T1, 4TO7, 167Farn and 67NR contains cancer stem cells defined by CXCR4 expression and their capability of forming spheroids in suspension culture. Importantly, we showed that CXCR4 expression is essential for tumor invasiveness because both CXCR4 neutralizing antibody and shRNA knockdown of the CXCR4 receptor significantly reduced tumor cell invasion.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Movement
  • Humans
  • In Vitro Techniques
  • Mammary Neoplasms, Animal / metabolism
  • Mice
  • Models, Biological
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Neoplastic Stem Cells
  • RNA, Small Interfering / metabolism
  • Receptors, CXCR4 / biosynthesis*
  • Receptors, CXCR4 / metabolism
  • Spheroids, Cellular / metabolism*
  • Tumor Cells, Cultured


  • RNA, Small Interfering
  • Receptors, CXCR4