Incorporation of an inducible nucleotide analog into DNA by DNA polymerases

Bioorg Med Chem. 2009 Apr 1;17(7):2859-63. doi: 10.1016/j.bmc.2009.02.024. Epub 2009 Feb 20.


Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5'-triphosphate of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA-Directed DNA Polymerase / metabolism*
  • Oligodeoxyribonucleotides / biosynthesis*
  • Oligodeoxyribonucleotides / chemistry
  • Purine Nucleotides / chemical synthesis*
  • Purine Nucleotides / chemistry
  • Templates, Genetic


  • 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine nucleoside triphosphate
  • Oligodeoxyribonucleotides
  • Purine Nucleotides
  • DNA-Directed DNA Polymerase