The Protein Expression of TRP-1 and galectin-1 in Cutaneous Malignant Melanomas

Cancer Genomics Proteomics. Nov-Dec 2008;5(6):293-300.

Abstract

Background: Patients with metastazing malignant melanoma have a poor outcome and determination of thickness of the primary tumor remains as the most important prognostic predictor. The aim of this study was to use an antibody-based proteomics strategy to search for new molecular markers associated with melanoma progression. Two proteins, TRP-1 and galectin-1, were identified as proteins with enhanced expression in cells from the melanocytic lineage.

Patients and methods: Protein profiling of TRP-1 and galectin-1 together with proliferation marker Ki-67 and melanocyte marker Melan-A was performed in normal tissues from 144 individuals and in 216 different tumors using tissue microarrays and immunohistochemistry. The protein expression pattern was further analyzed in a defined cohort of 157 patients diagnosed with invasive cutaneous malignant melanoma.

Results: Both TRP-1 and galectin-1 were highly expressed in normal melanocytes and melanoma. The expression of TRP-1 was inversely correlated with tumor stage (p=0.002, (R=-0.28)). Neither TRP-1 or galectin-1 was associated with overall or disease free survival (p>0.14, p>0.46 respectively). Ki-67 was associated with tumor stage and survival (p<0.001).

Conclusion: TRP-1 and galectin-1 protein expression patterns were determined in normal and cancer tissues and both proteins were expressed in the majority of the malignant melanomas. There was no correlation between TRP-1 or galectin-1 expression and survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Disease-Free Survival
  • Female
  • Follow-Up Studies
  • Galectin 1 / biosynthesis*
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Ki-67 Antigen / biosynthesis
  • Male
  • Melanocytes
  • Melanoma / metabolism*
  • Melanoma / mortality
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / metabolism
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Neoplasm Proteins / biosynthesis*
  • Oxidoreductases / biosynthesis*
  • Oxidoreductases / metabolism
  • Retrospective Studies
  • Skin Neoplasms / metabolism*
  • Skin Neoplasms / mortality
  • Survival Rate

Substances

  • Galectin 1
  • Ki-67 Antigen
  • LGALS1 protein, human
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • Oxidoreductases
  • TYRP1 protein, human