Human cord blood transplantation in a neonatal rat model of hypoxic-ischemic brain damage: functional outcome related to neuroprotection in the striatum

Stem Cells Dev. 2010 Mar;19(3):351-8. doi: 10.1089/scd.2009.0049.

Abstract

Human umbilical cord blood mononuclear cells (HUCB) have been shown to have a therapeutic role in different models of central nervous system (CNS) damage, including stroke. We evaluated the possible therapeutic potential of HUCB in P7 rats submitted to the Rice-Vannucci model of neonatal hypoxic-ischemic (HI) brain damage. Our results demonstrated that intraperitoneal transplantation of HUCB, 3 h after the HI insult, resulted in better performance in two developmental sensorimotor reflexes, in the first week after the injury. We also showed a neuroprotective effect in the striatum, and a decrease in the number of activated microglial cells in the cerebral cortex of treated animals. We suggest that HUCB transplantation might rescue striatal neurons from cell death after a neonatal HI injury resulting in better functional recovery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cord Blood Stem Cell Transplantation / methods*
  • Corpus Striatum / blood supply
  • Corpus Striatum / physiopathology
  • Corpus Striatum / surgery
  • Disease Models, Animal*
  • Humans
  • Hypoxia, Brain / etiology
  • Hypoxia, Brain / physiopathology
  • Hypoxia, Brain / surgery*
  • Hypoxia-Ischemia, Brain / complications
  • Hypoxia-Ischemia, Brain / prevention & control*
  • Infant, Newborn
  • Psychomotor Performance
  • Rats
  • Recovery of Function
  • Transplantation, Heterologous
  • Treatment Outcome