Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus

Genesis. 2009 May;47(5):300-4. doi: 10.1002/dvg.20499.

Abstract

We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Collagen / genetics
  • DNA, Helminth / genetics*
  • Genes, Dominant
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Helminth Proteins / genetics
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Molecular Sequence Data
  • Mutation*
  • Nematoda / genetics*
  • Plasmids / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Transformation, Genetic*
  • Transgenes / genetics

Substances

  • DNA, Helminth
  • Helminth Proteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • red fluorescent protein
  • Green Fluorescent Proteins
  • Collagen