Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment

Anal Chim Acta. 2009 Apr 6;638(1):51-7. doi: 10.1016/j.aca.2009.01.063. Epub 2009 Feb 8.


Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromatography, Affinity
  • Chromatography, Liquid
  • Mass Spectrometry
  • Microspheres*
  • Nanotechnology
  • Particle Size
  • Phosphopeptides / analysis*
  • Phosphopeptides / isolation & purification
  • Phosphorylation
  • Porosity
  • Tin Compounds / chemistry*


  • Phosphopeptides
  • Tin Compounds
  • stannic oxide