Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

Org Biomol Chem. 2009 Apr 7;7(7):1404-9. doi: 10.1039/b819946a. Epub 2009 Feb 24.


Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Catalysis
  • DNA / chemistry*
  • DNA / metabolism*
  • DNA-Directed DNA Polymerase / metabolism*
  • Molecular Sequence Data
  • Oligonucleotides / chemistry*
  • Oligonucleotides / metabolism*


  • Oligonucleotides
  • locked nucleic acid
  • DNA
  • DNA-Directed DNA Polymerase