Enzyme-linked immunosorbent assay for the detection and identification of plant pathogenic bacteria (in particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus)

Blanka Kokoskova; Jaap D Janse

doi:10.1007/978-1-59745-062-1_7

Enzyme-linked immunosorbent assay for the detection and identification of plant pathogenic bacteria (in particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus)

Methods Mol Biol. 2009:508:75-87. doi: 10.1007/978-1-59745-062-1_7.

Authors

Blanka Kokoskova¹, Jaap D Janse

Affiliation

¹ Department of Bacteriology, Crop Research Institute, Prague, Czech Republic.

PMID: 19301748
DOI: 10.1007/978-1-59745-062-1_7

Abstract

Enzyme-linked immunosorbent assay (ELISA) is the most commonly used serological diagnostic technique. A number of different ELISA formats can be used for the detection of bacterial plant pathogens and in particular Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus.

MeSH terms

Clinical Laboratory Techniques
Enzyme-Linked Immunosorbent Assay / methods*
Erwinia amylovora / isolation & purification*
Plant Diseases / microbiology*
Sensitivity and Specificity