Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli

Acta Virol. 2009;53(1):35-41. doi: 10.4149/av_2009_01_35.


Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142-1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein and a soluble NusA-HN protein containing N-utilization substance A (NusA) fusion component. Both recombinant proteins were purified and used for immunization of chickens. The recombinant HN protein induced higher antibody titers as compared to the recombinant NusA-HN protein. These antibodies were able to react in immunoblot analysis with the corresponding recombinant proteins as well as with the HN protein of NDV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Chickens
  • Cloning, Molecular
  • Escherichia coli
  • Gene Expression
  • HN Protein / immunology*
  • Newcastle disease virus / genetics
  • Newcastle disease virus / immunology*
  • Recombinant Proteins / immunology
  • Vaccines, Synthetic / immunology
  • Viral Proteins / immunology*
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology*


  • Antibodies, Viral
  • HN Protein
  • Recombinant Proteins
  • Vaccines, Synthetic
  • Viral Proteins
  • Viral Vaccines