In vivo activation of an omega-6 oxygenase in human skin

Biochem Biophys Res Commun. 1991 Oct 15;180(1):98-104. doi: 10.1016/s0006-291x(05)81260-4.


To test the hypothesis that an epidermal fatty acid oxygenase is activated in vivo under physiologic conditions, surface lipids from normal human skin were analyzed for oxygenase products. With high-performance liquid chromatography on reversed-phase and straight-phase chiral columns and gas-liquid chromatography/mass spectrometry, these lipids were found to contain free 13-hydroxyoctadeca-9Z,11E-dienoic acid and 9-hydroxyoctadeca-10E,12Z-dienoic acid. The 13-hydroxyoctadecadienoic acid was present as a stereoisomeric mixture, with an average S/R ratio of 2.2, and exceeded the concentration of 9-hydroxyoctadecadienoic acid by a factor of 2. These observations and others indicate that the 13-hydroxyoctadecadienoic acid was derived mostly from an omega-6 oxygenase (probably 15-lipoxygenase) which is activated in vivo in normal skin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antithrombins / metabolism
  • Arachidonate 15-Lipoxygenase / metabolism*
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Enzyme Activation
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Linoleic Acids / metabolism
  • Linoleic Acids, Conjugated*
  • Lipids / analysis
  • Male
  • Skin / chemistry
  • Skin / enzymology*
  • Stereoisomerism


  • Antithrombins
  • Linoleic Acids
  • Linoleic Acids, Conjugated
  • Lipids
  • 9-hydroxy-10,12-octadecadienoic acid
  • 13-hydroxy-9,11-octadecadienoic acid
  • Arachidonate 15-Lipoxygenase