Interaction of cis-diamminedichloroplatinum (II) with single-stranded DNA in the presence or absence of Escherichia coli single-stranded binding protein

Biochem Pharmacol. 1991 Sep 12;42(7):1393-8. doi: 10.1016/0006-2952(91)90450-j.


We have compared the mode of fixation in vitro of the antitumor drug cis-diamminedichloroplatinum (II) (cis-DDP) to single-stranded M13mp10 DNA either in the presence or absence of the Escherichia coli single-stranded binding protein (SSB). Platinum binding sites have been identified by taking advantage of their capacity to inhibit DNA replication of primed M13 DNA catalysed by E. coli DNA polymerase I large fragment. We report here that the presence of SSB increases the number of platinum-DNA lesions and alters their distribution. We also present evidence that SSB allows cis-DDP to bind to DNA sequences otherwise less accessible.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Binding Sites
  • Cisplatin / metabolism*
  • DNA Damage
  • DNA Replication / drug effects
  • DNA, Single-Stranded / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli*
  • Molecular Sequence Data
  • Molecular Structure
  • Polymerase Chain Reaction


  • Bacterial Proteins
  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • Cisplatin