Glucuronidation of imipramine in rabbit and human liver microsomes: assay conditions and interaction with other tertiary amine drugs

Biochem Pharmacol. 1991 Sep 12;42(7):1497-501. doi: 10.1016/0006-2952(91)90466-i.


The present work confirms that rabbit liver microsomes afford a good in vitro model for studying the glucuronidation of drugs containing tertiary amino groups. Furthermore, the availability of the assay procedure described above will allow us to understand further the basis for the inter-species differences in the metabolism of tertiary amines, and will eventually aid in our identification of the UDPGT isozyme(s) responsible for the conjugation of these compounds in man.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / metabolism
  • Animals
  • Detergents
  • Female
  • Glucuronates / metabolism*
  • Glucuronic Acid
  • Glucuronosyltransferase / metabolism*
  • Humans
  • Hydrogen-Ion Concentration
  • Imipramine / metabolism*
  • Male
  • Microsomes, Liver / metabolism*
  • Rabbits
  • Rats
  • Rats, Inbred Strains
  • Structure-Activity Relationship


  • Amines
  • Detergents
  • Glucuronates
  • Glucuronic Acid
  • Glucuronosyltransferase
  • Imipramine