A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Anal Biochem. 2009 Jun 1;389(1):89-91. doi: 10.1016/j.ab.2009.03.017. Epub 2009 Mar 19.


Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antibodies / chemistry*
  • Detergents / chemistry
  • Guanidine / chemistry
  • Hydrogen-Ion Concentration
  • Immunoblotting*
  • Polyvinyls / chemistry*
  • Temperature


  • Antibodies
  • Detergents
  • Polyvinyls
  • polyvinylidene fluoride
  • Guanidine