SUnSET, a nonradioactive method to monitor protein synthesis

Nat Methods. 2009 Apr;6(4):275-7. doi: 10.1038/nmeth.1314. Epub 2009 Mar 22.


We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Profiling / methods*
  • Immunoblotting / methods*
  • Isotope Labeling
  • Protein Biosynthesis / physiology*
  • Proteins / analysis*
  • Proteins / metabolism*
  • Puromycin / analysis*
  • Staining and Labeling / methods


  • Proteins
  • Puromycin