A human putative lymphocyte G0/G1 switch gene containing a CpG-rich island encodes a small basic protein with the potential to be phosphorylated

DNA Cell Biol. 1991 Oct;10(8):581-91. doi: 10.1089/dna.1991.10.581.

Abstract

Genes actively involved in the G0/G1 switch (G0S genes) may be differentially expressed during the lectin-induced switch of lymphocytes from the G0 to the G1 phases of the cell cycle. This paper presents studies of G0S2, a member of a set of putative G0S genes, for which cDNAs were cloned and selected on the basis of differential cDNA hybridization. G0S2 mRNA increases transiently within 1-2 hr of the addition of lectin or cycloheximide to cultured blood mononuclear cells. Comparison of a nearly full-length cDNA sequence with the corresponding genomic sequence reveals one small intron and an open reading frame in the second exon. The derived 103-amino-acid basic protein has two potential alpha-helical domains separated by a hydrophobic region with the potential to generate turns and assume a beta-sheet conformation. Consistent with involvement in the G0/G1 switch, the protein contains potential sites for phosphorylation by protein kinase C and casein kinase II. The gene contains a CpG-rich island suggesting expression in the germ line. An upstream segment contains tandem dinucleotide repeats (CT)19/(CA)16. There is a suitably located TATA box, but potential sites for CCAAT-box binding factors are far upstream, embedded in a 42-nucleotide repeat element. Potential sites for transcription factors AP1, AP2, and AP3 are consistent with rapid transcriptional activation in response to inducing agents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blood Proteins / genetics*
  • Blood Proteins / metabolism
  • DNA / genetics
  • Dinucleoside Phosphates / analysis*
  • Exons
  • Genes, Switch*
  • Humans
  • Hydrogen-Ion Concentration
  • Introns
  • Lymphocytes / chemistry*
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Conformation
  • RNA, Messenger / genetics
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • TATA Box
  • Transcription Factors / metabolism

Substances

  • Blood Proteins
  • Dinucleoside Phosphates
  • RNA, Messenger
  • Transcription Factors
  • cytidylyl-3'-5'-guanosine
  • DNA

Associated data

  • GENBANK/M69199
  • GENBANK/M72885
  • GENBANK/S59354
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/S64763
  • GENBANK/S64768
  • GENBANK/X64572
  • GENBANK/X64573
  • GENBANK/X64574