Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish

PLoS One. 2009;4(3):e4994. doi: 10.1371/journal.pone.0004994. Epub 2009 Mar 24.


The transcription factor Etsrp is required for vasculogenesis and primitive myelopoiesis in zebrafish. When ectopically expressed, etsrp is sufficient to induce the expression of many vascular and myeloid genes in zebrafish. The mammalian homolog of etsrp, ER71/Etv2, is also essential for vascular and hematopoietic development. To identify genes downstream of etsrp, gain-of-function experiments were performed for etsrp in zebrafish embryos followed by transcription profile analysis by microarray. Subsequent in vivo expression studies resulted in the identification of fourteen genes with blood and/or vascular expression, six of these being completely novel. Regulation of these genes by etsrp was confirmed by ectopic induction in etsrp overexpressing embryos and decreased expression in etsrp deficient embryos. Additional functional analysis of two newly discovered genes, hapln1b and sh3gl3, demonstrates their importance in embryonic vascular development. The results described here identify a group of genes downstream of etsrp likely to be critical for vascular and/or myeloid development.

MeSH terms

  • Animals
  • Blood Vessels / growth & development
  • Embryo, Nonmammalian
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Hematopoiesis / genetics*
  • Neovascularization, Physiologic / genetics*
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / physiology
  • Proteoglycans / genetics*
  • Proteoglycans / physiology
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Zebrafish
  • Zebrafish Proteins / genetics*
  • Zebrafish Proteins / physiology


  • Etv2 protein, zebrafish
  • Hapln1b protein, zebrafish
  • Nerve Tissue Proteins
  • Proteoglycans
  • Transcription Factors
  • Zebrafish Proteins
  • sh3gl3b protein, zebrafish