Direct radioactive labeling of unpurified PCR products using Klenow fragment

Biotechniques. 1991 Aug;11(2):164, 166.


We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Polymerase I*
  • Phosphorus Radioisotopes
  • Polymerase Chain Reaction / methods*
  • Repetitive Sequences, Nucleic Acid


  • Phosphorus Radioisotopes
  • DNA Polymerase I