Ectopic expression of sex peptide alters reproductive behavior of female D. melanogaster

Neuron. 1991 Oct;7(4):557-63. doi: 10.1016/0896-6273(91)90368-a.

Abstract

Sex peptide, a secreted component of the male accessory glands, has been shown to induce behavioral and physiological changes in mated Drosophila. We transformed flies with a hybrid gene containing an hsp70 promoter fused to a cDNA encoding sex peptide. Heat-induced ectopic expression of the peptide in transgenic virgin females altered their reproductive behavior, in the presence of courting males, to that observed in mated females. This demonstrates that the peptide is functional as expected. Time course studies revealed that the behavioral change appeared earlier than the stimulated ovulation. We have also introduced a modified sex peptide gene that is driven by the yp1 enhancer, conferring expression in adult females, and shown that these flies refuse mating constitutively in the presence of courting males and lay unfertilized eggs at the rate of mated females.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified / physiology
  • Diptera / genetics
  • Diptera / physiology
  • Drosophila Proteins*
  • Drosophila melanogaster / physiology*
  • Female
  • Hot Temperature
  • Intercellular Signaling Peptides and Proteins
  • Ovulation / physiology
  • Peptides / metabolism*
  • Sex Characteristics
  • Sexual Behavior, Animal / physiology*
  • Tissue Distribution

Substances

  • Acp36DE protein, Drosophila
  • Drosophila Proteins
  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • SP protein, Drosophila
  • male accessory gland peptide, Drosophila