High-throughput verification of transcriptional starting sites by Deep-RACE

Biotechniques. 2009 Feb;46(2):130-2. doi: 10.2144/000113066.


We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep-rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Base Sequence
  • DNA / genetics*
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques / methods*
  • Sequence Analysis, DNA / methods*
  • Transcription Factors / genetics*
  • Transcription Initiation Site*
  • Transcriptional Activation / genetics*


  • Transcription Factors
  • DNA