Homogeneous time-resolved fluoroimmunoassay for the measurement of midregional proadrenomedullin in plasma on the fully automated system B.R.A.H.M.S KRYPTOR

Pascaline Caruhel; Christian Mazier; Jan Kunde; Nils G Morgenthaler; Bruno Darbouret

doi:10.1016/j.clinbiochem.2009.01.002

Homogeneous time-resolved fluoroimmunoassay for the measurement of midregional proadrenomedullin in plasma on the fully automated system B.R.A.H.M.S KRYPTOR

Clin Biochem. 2009 May;42(7-8):725-8. doi: 10.1016/j.clinbiochem.2009.01.002. Epub 2009 Jan 23.

Authors

Pascaline Caruhel¹, Christian Mazier, Jan Kunde, Nils G Morgenthaler, Bruno Darbouret

Affiliation

¹ Research and Development Department, CEZANNE SAS, Parc Scientifique Georges Besse, Nimes Cedex 1, France. pcaruhel@cezanne.fr

PMID: 19318039
DOI: 10.1016/j.clinbiochem.2009.01.002

Abstract

Objectives: Evaluate the first automated assay measuring a mid regional fragment of the proadrenomedullin (MR-proADM).

Design and methods: B.R.A.H.M.S MR-proADM KRYPTOR is a homogeneous assay.

Results: In 144 healthy individuals, MR-proADM mean (SD) was measured at 0.37 nmol/L (0.09). High correlation with the manual assay, B.R.A.H.M.S MR-proADM LIA, was found on 281 samples from patients suffering from community acquired pneumonia (correlation coefficient 0.97).

Conclusions: This fast assay can be used in low respiratory tract infections.

MeSH terms

Adrenomedullin / blood*
Adult
Female
Fluoroimmunoassay / methods*
Humans
Male
Middle Aged
Protein Precursors / blood*
Reproducibility of Results
Young Adult

Substances

Protein Precursors
proadrenomedullin
Adrenomedullin