Relationship between gap size and dentine secondary caries formation assessed in a microcosm biofilm model

Caries Res. 2009;43(2):97-102. doi: 10.1159/000209341. Epub 2009 Mar 24.

Abstract

Secondary caries can develop at the tooth-restoration interface, depending on the presence of a gap and its size, but this process could be inhibited by fluoride. The aim of this study was to assess the relationship between gap size and dentine secondary caries adjacent to composite resin (CR) or glass ionomer (GI) restorations, using a microcosm biofilm model in a constant depth film fermentor (CDFF). Dentine discs restored with CR (Z250) or GI (Vitremer) with gap sizes of 0, 50, 100, 180 or 250 microm were mounted on the CDFF. Microcosm biofilms were formed on the restored discs and daily subjected to 8 pulses of 10% sucrose solution. On the 18th day, dentine mineral loss and lesion depth around the restorations were determined by transverse microradiography. The effect of gap size was overall not statistically significant either with regard to mineral loss (p = 0.449) or lesion depth (p = 0.328), but greater mineral loss and lesion depth were found adjacent to CR than to GI (p < 0.001). However, Spearman correlation showed that mineral loss and lesion depth increased with gap size for CR (p < 0.001) but not for GI (p > 0.05). The findings support the conclusion that fluoride released from GI inhibits dentine demineralization adjacent to restorations, irrespective of gap width.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriological Techniques
  • Biofilms*
  • Bisphenol A-Glycidyl Methacrylate / chemistry
  • Cariogenic Agents / metabolism
  • Cariostatic Agents / chemistry
  • Cattle
  • Composite Resins / chemistry
  • Dental Caries / etiology*
  • Dental Caries / microbiology
  • Dental Caries / pathology
  • Dental Marginal Adaptation*
  • Dental Materials / chemistry*
  • Dental Restoration, Permanent*
  • Dentin / microbiology
  • Dentin / ultrastructure*
  • Dentin-Bonding Agents / chemistry
  • Glass Ionomer Cements / chemistry
  • Microradiography
  • Random Allocation
  • Saliva / microbiology
  • Sucrose / metabolism
  • Surface Properties
  • Time Factors
  • Tooth Demineralization / etiology
  • Tooth Demineralization / microbiology
  • Tooth Demineralization / pathology

Substances

  • Cariogenic Agents
  • Cariostatic Agents
  • Composite Resins
  • Dental Materials
  • Dentin-Bonding Agents
  • Filtek Z250
  • Glass Ionomer Cements
  • Vitremer
  • single bond
  • Bisphenol A-Glycidyl Methacrylate
  • Sucrose