Anti-tumor activity of oxypeucedanin from Ostericum koreanum against human prostate carcinoma DU145 cells

Acta Oncol. 2009;48(6):895-900. doi: 10.1080/02841860902824925.


Purpose: Oxypeucedanin has been reported to have various biological activities. We investigated the efficacy of a coumarin compound, oxypeucedanin, from Ostericum koreanum against the human prostate carcinoma cell line DU145.

Material and methods: Oxypeucedanin (C(16)H(14)O(5), mw: 286) was isolated through silica gel chromatography and characterized by NMR. The cells were treated with oxypeucedanin (25, 50, and 100 microM) for 24-72 hours, and cell growth and death were then assayed. The cell cycle progression and apoptotic effects were also assessed by western blotting.

Results: Treatment with oxypeucedanin inhibited cell growth and induced cell death in DU145 cells. Furthermore, oxypeucedanin-induced cell growth inhibition was associated with an increase in G2-M arrest in cell cycle progression in DU145 cells in a dose and time-dependent manner. G2-M arrest by oxypeucedanin was associated with decreased levels of cyclin A, cyclin B1, Cdc2, and pCdc2. Oxypeucedanin-induced cell death was associated with significant increases in apoptosis and cleaved caspase-3 and poly-(ADP-ribose) polymerase.

Conclusion: These finding suggest a novel anticancer effect for oxypeucedanin, mediated via induction of G2-M cell cycle arrest and apoptosis in human prostate carcinoma DU145 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Apiaceae / chemistry*
  • Apoptosis / drug effects*
  • Caspase 3 / metabolism
  • Cell Division / drug effects
  • Cell Proliferation / drug effects
  • Cyclins / metabolism
  • Furocoumarins / isolation & purification
  • Furocoumarins / pharmacology*
  • G2 Phase / drug effects*
  • Humans
  • Immunoblotting
  • Male
  • Plant Roots / chemistry*
  • Poly(ADP-ribose) Polymerases / metabolism
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology*
  • Tumor Cells, Cultured


  • Antineoplastic Agents
  • Cyclins
  • Furocoumarins
  • Poly(ADP-ribose) Polymerases
  • Caspase 3
  • oxypeucadanin