[No-dependent modulation of the sensitivity of the mitochondrial permeability transition pore opening under ischemia-reperfusion of the isolated heart]

Ross Fiziol Zh Im I M Sechenova. 2009 Jan;95(1):28-37.
[Article in Russian]


The role of nitric oxide (NO) in modulation of sensitivity ofmitochondnal permeability transition pore (MPTP) opening under exposure to calcium as inductor was studied in experiments on the isolated heart (on Langendorff) and isolated mitochondria. The MPTP opening was studied on the hearts under ischemia-reperfusion and of the mitochondrial calcium loading. We investigated the degree ofreperfusional injury of the heart functional state after preliminary administration of the classic inhibitor of MPTP opening: cyclosporin A, the nitroprussid sodium salt as NO donor, NO-synthase (NOS) inhibitors such as L-NMMA and aminoguanidin to perfusional solution, and also the influence of Ca+ in the range of concentrations (10(-8)-10(-4) M) on the mitochondrial swelling under its preincubation with L-NMMA (10(-4) M). It is shown that the protective effect of nitric oxide on myocardium under reperfusion of ischemic heart will be realised by means of the inhibiting of Ca2+ -induced MPTP opening.

Publication types

  • English Abstract

MeSH terms

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / pharmacology
  • Animals
  • Calcium / metabolism
  • Cyclosporine / pharmacology
  • Dopamine Agents / pharmacology
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / pharmacology
  • Guinea Pigs
  • Male
  • Mitochondria, Heart / metabolism*
  • Mitochondrial Membranes / metabolism*
  • Myocardial Reperfusion Injury / metabolism*
  • Myocardium / metabolism*
  • Nitric Oxide / metabolism*
  • Nitric Oxide Donors / pharmacology
  • Nitroprusside / pharmacology
  • Rats
  • Rats, Wistar
  • Voltage-Dependent Anion Channels / metabolism*
  • omega-N-Methylarginine / pharmacology


  • Dopamine Agents
  • Enzyme Inhibitors
  • Nitric Oxide Donors
  • Voltage-Dependent Anion Channels
  • Nitroprusside
  • omega-N-Methylarginine
  • Nitric Oxide
  • Cyclosporine
  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
  • Calcium