Antioxidative activities of mushroom (Flammulina velutipes) extract added to bigeye tuna meat: dose-dependent efficacy and comparison with other biological antioxidants

J Food Sci. 2009 Mar;74(2):C162-9. doi: 10.1111/j.1750-3841.2009.01069.x.


The ability of a hydrophilic extract prepared from edible mushroom (Flammulina velutipes) to stabilize fresh color of bigeye tuna (Thunnus obesus) meat was evaluated to compare it with certain other antioxidants. The fresh color shelf life of bigeye tuna meats, to which were added as 1, 3, or 5 mL of mushroom extract to 100 g of minced bigeye tuna meat, prolonged duration of ice storage by more than 2, 4, and 6 d, respectively, in comparison with the control tuna meat without mushroom extract. The addition of 5 mL of mushroom extract to 100 g of minced bigeye tuna meat was more effective than adding ascorbic acid sodium salt (500 ppm) or alpha-tocopherol (500 ppm) with regard to oxidation of lipid in the tuna meat. The color changes significantly correlated with lipid oxidation as well as metmyoglobin formation in the tuna meat. These results clearly show that the mushroom extract is a potential antioxidant, which has the ability to stabilize fresh color of tuna meat during ice storage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / analysis*
  • Ergothioneine / analysis
  • Fatty Acids / analysis
  • Flammulina / chemistry*
  • Food Preservation
  • Hydrogen-Ion Concentration
  • Lipid Peroxides / analysis
  • Lipids / analysis
  • Meat / analysis*
  • Metmyoglobin / analysis
  • Muscle, Skeletal / chemistry
  • Plant Extracts / analysis*
  • Tuna


  • Antioxidants
  • Fatty Acids
  • Lipid Peroxides
  • Lipids
  • Plant Extracts
  • Metmyoglobin
  • Ergothioneine