One novel mitochondrial citrate synthase from Oryza sativa L. can enhance aluminum tolerance in transgenic tobacco

Mol Biotechnol. 2009 Jul;42(3):299-305. doi: 10.1007/s12033-009-9162-z. Epub 2009 Mar 27.

Abstract

Rice exhibits the greatest aluminum (Al) tolerance compared with other cereals such as wheat, barley, maize, etc. A full-length gene, OsCS1, encoding citrate synthase, which is highly induced by aluminum toxicity in rice (Oryza sativa L.), was isolated. Sequence analysis and the sub-cellular localization of OsCS1 in yeast revealed that it is a mitochondrial citrate synthase. OsCS1 was induced by Al toxicity. Several independent transgenic tobacco lines expressing OsCS 1 exhibited increased citrate efflux and extraordinary Al tolerance. Possible outlook for OsCS1 to be applied to enhance plant tolerance to Al toxicity was also discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum / pharmacology*
  • Amino Acid Sequence
  • Citrate (si)-Synthase / chemistry
  • Citrate (si)-Synthase / genetics
  • Citrate (si)-Synthase / metabolism*
  • Citric Acid / metabolism
  • Drug Tolerance
  • Gene Expression Regulation, Plant / drug effects
  • Mitochondria / enzymology*
  • Models, Molecular
  • Molecular Sequence Data
  • Oryza / drug effects*
  • Oryza / enzymology*
  • Oryza / genetics
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plant Roots / metabolism
  • Plants, Genetically Modified
  • Sequence Alignment

Substances

  • Plant Proteins
  • Citric Acid
  • Aluminum
  • Citrate (si)-Synthase