Effect of ovariectomy on the gene expression of detrusor muscarinic receptors in female rats

Cheng-Yu Long; Chun-Shuo Hsu; Pei-Lin Shao; Cheng-Min Liu; Juin-Huang Su; Eing-Mei Tsai

doi:10.1016/j.fertnstert.2009.01.161

Effect of ovariectomy on the gene expression of detrusor muscarinic receptors in female rats

Fertil Steril. 2009 Sep;92(3):1147-1149. doi: 10.1016/j.fertnstert.2009.01.161. Epub 2009 Mar 27.

Authors

Cheng-Yu Long¹, Chun-Shuo Hsu², Pei-Lin Shao³, Cheng-Min Liu³, Juin-Huang Su⁴, Eing-Mei Tsai⁵

Affiliations

¹ Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan.
² Department of Obstetrics and Gynecology, Buddhist Dalin Tzu Chi General Hospital, Chiayi, Taiwan.
³ Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan.
⁴ Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁵ Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: K83263@kmhk.kmu.edu.tw.

PMID: 19328480
DOI: 10.1016/j.fertnstert.2009.01.161

Abstract

Eight rats served as control subjects with sham surgeries and eight rats underwent ovariectomy. Two months later, the expression of muscarinic receptors, neuronal nitric oxide synthase (nNOS), and estrogen receptor (R) at the mRNA level were assessed by reverse-transcription polymerase chain reaction. The results showed that performing an ovariectomy in a virgin rat appears to have little effect on the gene expression of detrusor muscarinic receptors and urethral nNOS despite the menopausal events that occurred.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Estrogen Receptor beta / metabolism
Female
Gene Expression Regulation / physiology
Menopause / metabolism
Models, Animal
Muscle, Smooth / metabolism*
Nitric Oxide Synthase Type I / metabolism
Ovariectomy*
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Receptors, Muscarinic / genetics
Receptors, Muscarinic / metabolism*
Urethra / metabolism
Urinary Bladder / metabolism*
Urinary Bladder, Overactive / metabolism

Substances

Estrogen Receptor beta
RNA, Messenger
Receptors, Muscarinic
Nitric Oxide Synthase Type I