Layer-by-layer assembled gold nanoparticles for siRNA delivery

Nano Lett. 2009 May;9(5):2059-64. doi: 10.1021/nl9003865.


Although uptake into cells is highly complex and regulated, heterogeneous particle collectives are usually employed to deliver small interfering RNA (siRNA) to cells. Within these collectives, it is difficult to accurately identify the active species, and a decrease in efficacy is inherent to such preparations. Here, we demonstrate the manufacture of uniform nanoparticles with the deposition of siRNA on gold in a layer-by-layer approach, and we further report on the cellular delivery and siRNA activity as functions of surface properties.

MeSH terms

  • Animals
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Gold / chemistry*
  • Green Fluorescent Proteins / antagonists & inhibitors
  • Green Fluorescent Proteins / genetics
  • Metal Nanoparticles / chemistry*
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism*
  • Transfection*


  • RNA, Small Interfering
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Gold