Stimulation of guanylyl cyclase-D by bicarbonate

Biochemistry. 2009 May 26;48(20):4417-22. doi: 10.1021/bi900441v.


Guanylyl cyclases (GCs) catalyze the conversion of GTP to the second messenger cGMP. While some transmembrane GCs are receptors for extracellular ligands, other transmembrane GCs such as retinal-specific GC-E and GC-F are stimulated by cellular proteins. GC-D is expressed in a special group of olfactory sensory neurons. However, the direct regulatory mechanism of GC-D activity is not completely understood. Here we have demonstrated that bicarbonate directly increases the activity of purified GC-D. Bicarbonate also increases the cGMP levels in cells expressing GC-D. These results identify bicarbonate as a small molecule that regulates GC-D.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Bicarbonates / chemistry*
  • CHO Cells
  • Cell Membrane / metabolism
  • Cricetinae
  • Cricetulus
  • Cyclic GMP / metabolism
  • Glutathione Transferase / metabolism
  • Guanosine Triphosphate / chemistry
  • Guanylate Cyclase / chemistry*
  • Guanylate Cyclase / metabolism
  • Ligands
  • Olfactory Receptor Neurons / metabolism*
  • Plasmids / metabolism
  • Protein Structure, Tertiary
  • Transfection


  • Bicarbonates
  • Ligands
  • Guanosine Triphosphate
  • Glutathione Transferase
  • Guanylate Cyclase
  • Cyclic GMP