TRPV3, a thermosensitive channel is expressed in mouse distal colon epithelium

Biochem Biophys Res Commun. 2009 May 22;383(1):130-4. doi: 10.1016/j.bbrc.2009.03.143. Epub 2009 Mar 29.


The thermo-transient receptor potential (thermoTRP) subfamily is composed of channels that are important in nociception and thermo-sensing. Here, we show a selective expression of TRPV3 channel in the distal colon throughout the gastrointestinal tract. Expression analyses clearly revealed that TRPV3 mRNA and proteins were expressed in the superficial epithelial cells of the distal colon, but not in those of the stomach, duodenum or proximal colon. In a subset of primary epithelial cells cultured from the distal colon, carvacrol, an agonist for TRPV3, elevated cytosolic Ca(2+)concentration in a concentration-dependent manner. This response was inhibited by ruthenium red, a TRPV channel antagonist. Organotypic culture supported that the carvacrol-responsive cells were present in superficial epithelial cells. Moreover, application of carvacrol evoked ATP release in primary colonic epithelial cells. We conclude that TRPV3 is present in absorptive cells in the distal colon and may be involved in a variety of cellular functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Colon / cytology
  • Colon / drug effects
  • Colon / metabolism*
  • Cymenes
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Monoterpenes / pharmacology
  • TRPV Cation Channels / biosynthesis*


  • Cymenes
  • Monoterpenes
  • TRPV Cation Channels
  • Trpv3 protein, mouse
  • Adenosine Triphosphate
  • carvacrol