Dicer-1-dependent Dacapo suppression acts downstream of Insulin receptor in regulating cell division of Drosophila germline stem cells

Development. 2009 May;136(9):1497-507. doi: 10.1242/dev.025999. Epub 2009 Mar 31.

Abstract

It is important to understand the regulation of stem cell division because defects in this process can cause altered tissue homeostasis or cancer. The cyclin-dependent kinase inhibitor Dacapo (Dap), a p21/p27 homolog, acts downstream of the microRNA (miRNA) pathway to regulate the cell cycle in Drosophila melanogaster germline stem cells (GSCs). Tissue-extrinsic signals, including insulin, also regulate cell division of GSCs. We report that intrinsic and extrinsic regulators intersect in GSC division control; the Insulin receptor (InR) pathway regulates Dap levels through miRNAs, thereby controlling GSC division. Using GFP-dap 3'UTR sensors in vivo, we show that in GSCs the dap 3'UTR is responsive to Dicer-1, an RNA endonuclease III required for miRNA processing. Furthermore, the dap 3'UTR can be directly targeted by miR-7, miR-278 and miR-309 in luciferase assays. Consistent with this, miR-278 and miR-7 mutant GSCs are partially defective in GSC division and show abnormal cell cycle marker expression, respectively. These data suggest that the GSC cell cycle is regulated via the dap 3'UTR by multiple miRNAs. Furthermore, the GFP-dap 3'UTR sensors respond to InR but not to TGF-beta signaling, suggesting that InR signaling utilizes Dap for GSC cell cycle regulation. We further demonstrate that the miRNA-based Dap regulation may act downstream of InR signaling; Dcr-1 and Dap are required for nutrition-dependent cell cycle regulation in GSCs and reduction of dap partially rescues the cell cycle defect of InR-deficient GSCs. These data suggest that miRNA- and Dap-based cell cycle regulation in GSCs can be controlled by InR signaling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Animals
  • Animals, Genetically Modified
  • Cell Division
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism*
  • Gene Expression Regulation
  • Gene Expression Regulation, Developmental
  • Germ Cells / cytology
  • Germ Cells / metabolism*
  • MicroRNAs / genetics
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • RNA Helicases / genetics
  • RNA Helicases / metabolism*
  • Receptor, Insulin / genetics
  • Receptor, Insulin / metabolism*
  • Ribonuclease III
  • Signal Transduction
  • Stem Cells / cytology*
  • Stem Cells / metabolism*
  • Transforming Growth Factor beta / metabolism

Substances

  • 3' Untranslated Regions
  • Drosophila Proteins
  • MicroRNAs
  • Nuclear Proteins
  • Transforming Growth Factor beta
  • dap protein, Drosophila
  • Receptor, Insulin
  • Dcr-1 protein, Drosophila
  • Ribonuclease III
  • RNA Helicases