Analysis of Msx1 and Msx2 transactivation function in the context of the heat shock 70 (Hspa1b) gene promoter

Biochem Biophys Res Commun. 2009 Apr 3;381(2):241-6. doi: 10.1016/j.bbrc.2009.02.016. Epub 2009 Feb 11.

Abstract

Previous studies have shown that Msx proteins control gene transcription predominantly through repression mechanisms. However, gene expression studies using either the gain-of-function or the loss-of-function mutants revealed many gene targets whose expression require functional Msx proteins. To date, investigations into the mechanisms of Msx-dependent transactivation have been hindered by the lack of a responsive promoter. Here, we demonstrated the usefulness of the mouse Hspa1b promoter in probing Msx-dependent mechanisms of gene activation. We showed that Msx protein activates Hspa1b promoter via its C-terminal domain. The activation absolutely depends on the HSEs and physical interactions between Msx proteins and heat shock factors may play a contributing role.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • HSP70 Heat-Shock Proteins / genetics*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • MSX1 Transcription Factor / genetics
  • MSX1 Transcription Factor / metabolism*
  • Mice
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Response Elements
  • Transcriptional Activation*

Substances

  • HSP70 Heat-Shock Proteins
  • Homeodomain Proteins
  • MSX1 Transcription Factor
  • MSX2 protein
  • Msx1 protein, mouse
  • heat-shock protein 70.1