p53 gene mutations and abnormal retinoblastoma protein in radiation-induced human sarcomas

Cancer Res. 1991 Dec 1;51(23 Pt 1):6393-6.


The potentially carcinogenic effect of therapeutic irradiation has been recognized for many years. Second malignancies, usually sarcomas, are known to arise within or at the edge of radiation fields after a period of several years after the initial radiation exposure. We analyzed tumor cells derived from seven radiation-induced tumors for abnormalities in tumor suppressor genes p53 and retinoblastoma at the DNA sequence and/or protein level. p53 mutations were detected by exon-specific polymerase chain reaction amplification and single-strand conformation polymorphism analysis of exons 5-8 followed by direct genomic sequencing of those tumors exhibiting a variant pattern. The p53 gene was abnormal in three of six sarcomas studied. Retinoblastoma gene analysis was performed by Western immunoblot; retinoblastoma protein was under-phosphorylated in three of seven tumors and absent in one other. In all, six of seven radiation-induced human tumors have abnormalities of one or both suppressor genes. Inactivation of tumor suppressor genes by ionizing radiation may contribute to radiation carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Exons / genetics*
  • Genes, Retinoblastoma / genetics*
  • Genes, p53 / genetics*
  • Molecular Sequence Data
  • Mutation / genetics*
  • Neoplasms, Radiation-Induced / genetics*
  • Polymerase Chain Reaction
  • Retinoblastoma Protein / genetics*
  • Sarcoma / genetics*
  • Tumor Cells, Cultured


  • Retinoblastoma Protein

Associated data

  • GENBANK/S63143
  • GENBANK/S63144
  • GENBANK/S63145
  • GENBANK/S63146
  • GENBANK/S63147
  • GENBANK/S63148
  • GENBANK/S66573
  • GENBANK/S66577
  • GENBANK/S66615
  • GENBANK/X62953