Three-dimensional noninvasive imaging of the vasculature in the mouse brain using a high resolution photoacoustic scanner

Appl Opt. 2009 Apr 1;48(10):D299-306. doi: 10.1364/ao.48.00d299.


The application of a novel photoacoustic imaging instrument based on a Fabry-Perot polymer film sensing interferometer to imaging the small animal brain is described. This approach provides a convenient backward mode sensing configuration that offers the prospect of overcoming the limitations of existing piezoelectric based detection schemes for small animal brain imaging. Noninvasive images of the vasculature in the mouse brain were obtained at different wavelengths between 590 and 889 nm, showing that the cerebral vascular anatomy can be visualized with high contrast and spatial resolution to depths up to 3.7 mm. It is considered that the instrument has a role to play in characterizing small animal models of human disease and injury processes such as stroke, epilepsy, and traumatic brain injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Vessels / anatomy & histology
  • Blood Vessels / pathology
  • Brain / blood supply*
  • Imaging, Three-Dimensional / instrumentation*
  • Imaging, Three-Dimensional / methods*
  • Interferometry / instrumentation
  • Mice
  • Optical Devices*
  • Ultrasonics*