Human mesenchymal stem cells (hMSCs) target osteosarcoma and promote its growth and pulmonary metastasis

Cancer Lett. 2009 Aug 18;281(1):32-41. doi: 10.1016/j.canlet.2009.02.022. Epub 2009 Apr 1.


In an effort to study the interaction between MSCs and osteosarcoma, we established an animal model of primary osteosarcoma in nude mice using Saos-2 cells. hMSCs, labeled with adv-GFP, were injected through the caudal vein. We observed that exogenous hMSCs targeted the osteosarcoma site and promoted its growth and pulmonary metastasis in vivo. To elucidate the underlying mechanisms, we employed transwell, neutralization antibody and MTT assays in vitro. hMSCs migrated toward the conditioned medium from Saos-2 cells, and SDF-1 was involved in this migration. Likewise, Saos-2 cells migrated toward the conditioned medium from hMSCs and CCL5 played an important role in this migration. Furthermore, proliferation of Saos-2 cells was enhanced by the conditioned medium from hMSCs and CCL5 was at least partly responsible for this enhancement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Neoplasms / metabolism
  • Bone Neoplasms / pathology*
  • Cell Line, Tumor / metabolism
  • Cell Line, Tumor / transplantation
  • Cell Movement
  • Chemokine CCL5 / metabolism
  • Chemokine CCL5 / physiology*
  • Chemokine CXCL12 / metabolism
  • Chemokine CXCL12 / physiology*
  • Culture Media, Conditioned / pharmacology
  • Disease Progression
  • Humans
  • Lung Neoplasms / secondary*
  • Male
  • Mesenchymal Stem Cell Transplantation / adverse effects*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Osteosarcoma / metabolism
  • Osteosarcoma / pathology*
  • Osteosarcoma / secondary


  • CCL5 protein, human
  • CXCL12 protein, human
  • Chemokine CCL5
  • Chemokine CXCL12
  • Culture Media, Conditioned