Surface phenotype and rapid quantification of blood dendritic cell subsets in the rhesus macaque

J Med Primatol. 2009 Aug;38(4):272-8. doi: 10.1111/j.1600-0684.2009.00353.x. Epub 2009 Mar 31.


Background: The study of dendritic cell (DC) biology in the rhesus macaque is becoming increasingly important but is limited by incomplete characterization and the lack of a rapid assay to quantify cells.

Methods: We characterized the surface phenotype of myeloid (mDC) and plasmacytoid DC (pDC) subsets in healthy rhesus macaque blood and developed a flow cytometry-based assay for absolute DC determinations.

Results: Rhesus CD11c(+) mDC were CD16(+) CD11b(+) CD56(lo) CD8(-) CD1c(-) whereas CD123(+) pDC lacked expression of these markers. Precise DC determinations were performed using a rapid two-step assay combining the analysis of whole blood and peripheral blood leukocytes (PBL).

Conclusions: Antibodies to CD11b, CD56 and CD16 must be omitted from the lineage antibody cocktail to prevent inadvertent gating-out of DC when analyzing rhesus blood. The combined whole-blood/PBL quantification assay will be invaluable for the rapid and repeated monitoring of blood DC counts in this species.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigens, Surface / metabolism
  • Blood Cell Count / instrumentation
  • Blood Cell Count / methods
  • Blood Cell Count / veterinary*
  • Dendritic Cells / classification*
  • Dendritic Cells / physiology*
  • Macaca mulatta / blood*


  • Antigens, Surface