Compound MMH01 possesses toxicity against human leukemia and pancreatic cancer cells

Toxicol In Vitro. 2009 Apr;23(3):418-24. doi: 10.1016/j.tiv.2009.01.011. Epub 2009 Jan 22.


MMH01 is a compound isolated from Antrodia cinnamomea. MMH01 markedly inhibited growth of human leukemia U937 and pancreatic cancer BxPC3 cells. It resulted in distinct patterns of cell cycle distribution in U937 (G2/M, sub-G1 and polyploidy) and BxPC3 cells (G0/G1 and sub-G1). The modes of cell death in U937 cells include apoptosis and mitotic catastrophe, whereas apoptosis-associated events or necrosis in BxPC3 cells. Neither mitochondrial membrane permeabilization nor caspase dependence was noted. Proteins involving mitotic catastrophe-associated cell death such as cyclin B1 and checkpoint kinase 2 were activated in U937 cells. Only slight to moderate viability inhibition was noted to human monocytes, the normal counterpart of these myeloid leukemic cells. In conclusion, MMH01 possesses cytotoxicity against human leukemia and pancreatic cancer cells.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Antrodia / chemistry*
  • Cell Cycle / drug effects
  • Cell Survival / drug effects
  • Drug Screening Assays, Antitumor
  • Humans
  • Lanosterol / analogs & derivatives*
  • Lanosterol / isolation & purification
  • Lanosterol / pharmacology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / pathology
  • Lymphoma, Large B-Cell, Diffuse / drug therapy*
  • Lymphoma, Large B-Cell, Diffuse / pathology
  • Membrane Potential, Mitochondrial / drug effects
  • Pancreatic Neoplasms / drug therapy*
  • Pancreatic Neoplasms / pathology
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Plants, Medicinal / chemistry*
  • Taiwan
  • U937 Cells


  • 24-methylenelanosta-7,9(11)-diene-3,15-diol-21-oic acid
  • Antineoplastic Agents
  • Plant Extracts
  • Lanosterol