Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate

Biochem Biophys Res Commun. 2009 May 22;383(1):151-5. doi: 10.1016/j.bbrc.2009.03.145. Epub 2009 Apr 1.


Tobacco smoking is an important cause of human oral squamous cell carcinoma (SCC). Tobacco smoke contains multiple carcinogens include polycyclic aromatic hydrocarbons typified by benzo[a]pyrene (B[a]P). Surgery is the conventional treatment approach for SCC, but it remains imperfect. However, chemoprevention is a plausible strategy and we had previously demonstrated that 1,4-phenylenebis(methylene)selenocyanate (p-XSC) significantly inhibited tongue tumors-induced by the synthetic 4-nitroquinoline-N-oxide (not present in tobacco smoke). In this study, we demonstrated that p-XSC is capable of inhibiting B[a]P-DNA adduct formation, cell proliferation, cyclin D1 expression in human oral cells in vitro. In addition, we showed that dietary p-XSC inhibits B[a]P-DNA adduct formation, cell proliferation and cyclin D1 protein expression in the mouse tongue in vivo. The results of this study are encouraging to further evaluate the chemopreventive efficacy of p-XSC initially against B[a]P-induced tongue tumors in mice and ultimately in the clinic.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anticarcinogenic Agents / pharmacology*
  • Benzo(a)pyrene / antagonists & inhibitors*
  • Benzo(a)pyrene / toxicity
  • Carcinogens / antagonists & inhibitors*
  • Carcinogens / toxicity
  • Cell Line, Tumor
  • Cell Survival
  • Cell Transformation, Neoplastic / drug effects*
  • Cyclin D1 / metabolism
  • DNA Adducts / metabolism
  • Humans
  • Mouth / drug effects*
  • Mouth / metabolism
  • Mouth / pathology
  • Organoselenium Compounds / pharmacology*
  • Proliferating Cell Nuclear Antigen / metabolism


  • Anticarcinogenic Agents
  • Carcinogens
  • DNA Adducts
  • Organoselenium Compounds
  • Proliferating Cell Nuclear Antigen
  • Cyclin D1
  • Benzo(a)pyrene
  • 1,4-phenylenebis(methylene)selenocyanate