Recombinant bispecific single chain antibody fragments induce Fc gamma-receptor-mediated elimination of CD30+ lymphoma cells

Cancer Lett. 2009 Sep 18;282(2):187-94. doi: 10.1016/j.canlet.2009.03.011. Epub 2009 Apr 3.


Bispecific molecules (BSMs) facilitate the targeting of immune effector cells to tumor cells. Here we describe the construction and characterization of a recombinant BSM comprising two single chain fragments: H22(scFv), targeting the Fc gamma-receptor (CD64) on monocytes, and Ki4(scFv), targeting CD30 on Hodgkin lymphoma cells. A homologous, chemically-linked BSM has been described previously, but is heterogeneous and difficult to prepare. The recombinant version is easier to prepare and homogeneous, yet retains the antigen specificities and efficiently triggers CD64-related effector functions. The elimination of lymphoma cells was preferentially achieved by phagocytosis, not through the ADCC pathway additionally activated by the chemically-linked molecule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bispecific / therapeutic use*
  • Antibody-Dependent Cell Cytotoxicity
  • Cell Line
  • Cloning, Molecular
  • Hodgkin Disease / immunology
  • Hodgkin Disease / therapy*
  • Humans
  • Immunoglobulin Fragments / therapeutic use*
  • Ki-1 Antigen / analysis
  • Ki-1 Antigen / immunology*
  • Phagocytosis
  • Receptors, IgG / immunology*
  • Recombinant Proteins / therapeutic use


  • Antibodies, Bispecific
  • Immunoglobulin Fragments
  • Ki-1 Antigen
  • Receptors, IgG
  • Recombinant Proteins
  • immunoglobulin Fv