Isolation and enrichment of stem cells

Adv Biochem Eng Biotechnol. 2009:114:23-72. doi: 10.1007/10_2008_38.


Stem cells have the potential to revolutionize tissue regeneration and engineering. Both general types of stem cells, those with pluripotent differentiation potential as well as those with multipotent differentiation potential, are of equal interest. They are important tools to further understanding of general cellular processes, to refine industrial applications for drug target discovery and predictive toxicology, and to gain more insights into their potential for tissue regeneration. This chapter provides an overview of existing sorting technologies and protocols, outlines the phenotypic characteristics of a number of different stem cells, and summarizes their potential clinical applications.

Publication types

  • Review

MeSH terms

  • Adult
  • Adult Stem Cells / cytology*
  • Adult Stem Cells / physiology
  • Cell Differentiation
  • Cells, Cultured
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / physiology
  • Flow Cytometry
  • Guided Tissue Regeneration
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / physiology
  • Humans
  • Immunomagnetic Separation / methods*
  • Multipotent Stem Cells / cytology*
  • Multipotent Stem Cells / physiology
  • Tissue Engineering
  • Transplantation, Homologous