Rpi-vnt1.1, a Tm-2(2) Homolog From Solanum Venturii, Confers Resistance to Potato Late Blight

Mol Plant Microbe Interact. 2009 May;22(5):589-600. doi: 10.1094/MPMI-22-5-0589.

Abstract

Despite the efforts of breeders and the extensive use of fungicide control measures, late blight still remains a major threat to potato cultivation worldwide. The introduction of genetic resistance into cultivated potato is considered a valuable method to achieve durable resistance to late blight. Here, we report the identification and cloning of Rpi-vnt1.1, a previously uncharacterized late-blight resistance gene from Solanum venturii. The gene was identified by a classical genetic and physical mapping approach and encodes a coiled-coil nucleotide-binding leucine-rich repeat protein with high similarity to Tm-2(2) from S. lycopersicum which confers resistance against Tomato mosaic virus. Transgenic potato and tomato plants carrying Rpi-vnt1.1 were shown to be resistant to Phytophthora infestans. Of 11 P. infestans isolates tested, only isolate EC1 from Ecuador was able to overcome Rpi-vnt1.1 and cause disease on the inoculated plants. Alleles of Rpi-vnt1.1 (Rpi-vnt1.2 and Rpi-vnt1.3) that differed by only a few nucleotides were found in other late-blight-resistant accessions of S. venturii. The late blight resistance gene Rpi-phu1 from S. phureja is shown here to be identical to Rpi-vnt1.1, suggesting either that this strong resistance gene has been maintained since a common ancestor, due to selection pressure for blight resistance, or that genetic exchange between S. venturii and S. phureja has occurred at some time.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Chromosomes, Plant / genetics
  • Cloning, Molecular
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • Genes, Plant / genetics*
  • Host-Pathogen Interactions
  • Immunity, Innate / genetics
  • Lycopersicon esculentum / genetics
  • Lycopersicon esculentum / microbiology
  • Molecular Sequence Data
  • Phytophthora infestans / physiology
  • Plant Diseases / genetics*
  • Plant Diseases / microbiology
  • Plant Proteins / genetics*
  • Plants, Genetically Modified / genetics*
  • Plants, Genetically Modified / microbiology
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Solanum / classification
  • Solanum / genetics*
  • Solanum / microbiology
  • Solanum tuberosum / genetics
  • Solanum tuberosum / microbiology
  • Species Specificity

Substances

  • DNA, Plant
  • Plant Proteins

Associated data

  • GENBANK/FJ423044
  • GENBANK/FJ423045
  • GENBANK/FJ423046